Characterization of ATP Binding in γ-Glutamylcysteine Synthetase

Computational and spectroscopic techniques have been combined to probe local protein structure. Experimental resonance Raman spectra were obtained for small fragments of the 29 amino acid glucose regulating protein glucagon. The Raman spectra for these fragments were also simulated computationally and were used to aid in the interpretation of the experimental spectra. Glucagon was then probed with experimental resonance Raman at a wavelength of 224 nm. Both the experimental and computational data from the smaller fragments were used to interpret the full glucagon spectrum.